Chitin, a polymer of N-acetyl-D-glucosamine (GlcNAc), serves as a key structural component of crustaceans, insects and fungi and is a polysaccharide second most abundant in nature. Although chitin-containing organism has been suggested as a source of novel Chicken Clia Kits animal feed, chitin has long been regarded as indigestible fiber in the animal body. More recently, we reported that the acidic chitinase (Chia) is the main glycosidase protease resistant rat gastrointestinal tract (GIT) and digest chitin in rat stomach. However, the physiological role of Chia in other animals including poultry still unknown. Here, we report that Chia can function as digestive enzymes that break down chitin-containing organism chicken GIT. Chia mRNA is mainly expressed in the glandular stomach tissue in normal chickens. We also showed that chicken Chia has chitinolytic strong activity at pH 2.0 and very resistant to proteolysis by pepsin and trypsin / chymotrypsin under conditions ...
Chitin digestibility is dependent on feeding behaviors, which determine acidic chitinase mRNA levels in mammalian and poultry stomachs.
Chitin, a polymer of N-acetyl-D-glucosamine (GlcNAc), serves as a key structural component of chitin-containing organisms including crustaceans, insects and fungi. More recently, we reported that the acidic chitinase (Chia) is highly expressed in mouse , chicken Equine Clia Kits and pork belly network and that it can digest chitin in their gastrointestinal tract (GIT). In this study, we focus on major livestock and domestic animals and showed that the mRNA levels of Chia in their stomach tissue is regulated by feeding behavior. Chia mRNA levels were significantly lower in cows (herbivores) and dogs (carnivores) of the stomach compared with rats, pigs and chicken (omnivores). Consistent with the level of mRNA, protein Chia is very low in the stomach of a cow. In addition, chitinolytic activity of E. coli-expressed enzyme Chia cows and dogs are pretty but significantly lower than those of the enzyme Chia omnivores. cows and dogs Chia recombinant enzyme can degrade chiti...